FLOW CYTOMETRY CORE FACILITIES AT UC BERKELEY

Laser

Parameter

Filters

Fluorochromes

488 nm

FL 1
FL2

 550 LP        530/40 BP
 550 LP        710/40 BP

FITC, GFP, YFP, CFSE
PerCP Cy 5.5, PI

405 nm

FL 8
FL 9

 480 LP        460/50 BP
 480 LP        550/50 BP

Pacific Blue
Pacific Orange, AmCyan

UV

FL 6
FL 7

 550 LP        460/50 BP
 550 LP        670/30 BP

Hoechst, DHPI
Red Hoechst

561 nm

FL 3
FL 4
FL 5

 645 LP        593/40 BP
 700 LP        670/30 BP
 700 LP        750 LP

PE, M-Cherry, RFP
PECy5
PECy7

640 nm

FL 10
FL 11

 700 LP        660/20 BP
 700 LP        750 LP

APC
APC Cy7